{"text_format":"1","workbook":"xlsx","n_sheets":4,"sheets":["infos échantillons","Ressource","Version","Feuil1"],"not_rendered":{"charts":0,"images":0,"macros":0,"external_links":0,"defined_names":10,"comments":1,"pivot_tables":0},"unopened":[],"bounds":{"document_bytes":16777216,"sheet_cells":100000,"rows_first":2000,"rows_last":500,"rows_between":2000,"halvings":0,"cell_chars":16384,"cell_head_chars":4096},"cells_cut":0,"cells_raw":0,"read":"whole","date_system":"1900"}

{"sheet":"infos échantillons","index":0,"n_rows":2815,"n_cols":16,"rows_kept":2815,"cut":false,"hidden":false,"merged":[[0,4,0,9],[9,1,9,4],[0,0,0,3],[23,1,23,2],[15,1,15,2],[2,3,2,4],[2,1,2,2],[2,6,3,10],[20,1,20,2],[16,1,16,2],[17,1,17,2],[18,1,18,2],[19,1,19,2],[8,6,8,10],[24,1,24,2],[29,0,29,15],[13,1,13,3],[14,1,14,3],[7,6,7,10],[26,1,26,2],[27,1,27,2],[25,1,25,2],[22,1,22,2],[21,1,21,2]]}
["Fiche échantillon pour le projet  : "]
[]
[null,"Pré-requis ARN totaux avec une purif poly-A (séquençage des ARN messagers)",null,"Pré-requis ARN sans purif poly-A (ribodéplétés, séquençage des ARN totaux, ARN amplifiés…)",null,null,"Merci de ne pas modifier ni l'emplacement des colonnes ni leurs titres"]
[null,"Qté min. de matrice :","5µg","Qté min. de matrice :","300ng"]
[null,"concentration min. :","100 ng/µl","concentration min. :","50 ng/µl"]
[null,"Pureté 260/280 :","1,8","Pureté 260/280 :","1,8",null,"a si vos échantillons ne valident pas les pré-requis, contacter la plateforme"]
[null,"Pureté 260/230 :","1,8","Pureté 260/230 :","1,8"]
[null,"BioAnalyzer :","RIN>8,5 ; 28S/18S>1,7*","BioAnalyzer :","RIN>8,5 ; 28S/18S>1,7*",null,"* Si le support de vos échantillons (tube, plaque) a un code-barres, merci de le spécifier dans la colonne Code-barres"]
[null,"Volume minimum: ","20µl","Volume minimum: ","8µl",null,"* Choisir des noms d'échantillons courts sans caractères spéciaux (espaces, virgules, points, underscores …) sauf tiret toléré"]
[null,"*à adapter selon espèce et projet (Procaryotes : 23S/16S>1,2), joindre les profils BioAnalyzer",null,null,null,null,"* Vérifier que le nom sur le tube correspond au nom inscrit dans le fichier"]
[null,null,null,null,null,null,"* Afin de réaliser les analyses qualité de vos données, merci de nous fournir le lien web vers le génome et/ou transcriptome de référence et sa version"]
[null,"Consultez le document Prérequis pour plus d'information"]
[]
[null,"A renseigner par l'équipe de recherche"]
[]
[null,"Nature de la matrice",null,"TRANSCRIPTOMIC",{"$f":"IFERROR(INDEX(Ressource!A3:B11,MATCH(D16,Ressource!A3:A11,0),2),\"\")","v":" Transcription products or non genomic DNA (EST, cDNA, RT-PCR, screened libraries)."}]
[null,"Type de librairie",null,null,{"$f":"IFERROR(INDEX(Ressource!E3:F38,MATCH(D17,Ressource!E3:E38,0),2),\"\")","v":""}]
[null,"Sélection de librairie",null,null,{"$f":"IFERROR(INDEX(Ressource!C3:D33,MATCH(D18,Ressource!C3:C33,0),2),\"\")","v":""}]
[null,"Espèce (nom scientifique)"]
[null,"Méthode d'extraction"]
[null,"Elution"]
[null,"Nombre d'échantillons"]
[null,"Nombre de lanes"]
[null,"Multiplexage",null,"n-plex / non"]
[null,"Conserver mes échantillons",null,"Non"]
[]
[null,"Identifiant/lien Génome de référence ( NCBI/Ensembl…)"]
[null,"Identifiant/lien Transcriptome de référence ( NCBI/Ensembl…)"]
[]
["Informations sur les échantillons à renseigner par l'équipe de recherche"]
[null,"Support","puits sur plaque","Code-barres","Nom Echantillon","Espèce (nom scientifique)","Estimation concentration nanodrop (ng/µl)","ratio 260/280","ratio 260/230","RIN","Dosage au QuBit  ou Picogreen (ng/µl)","Volume (µl)","Quantité fournie (µg)","Nature de la matrice","Type de librairie","Sélection de librairie"]
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{"sheet":"Ressource","index":1,"n_rows":38,"n_cols":6,"rows_kept":38,"cut":false,"hidden":false,"merged":[[0,0,0,1],[0,2,0,3],[0,4,0,5]]}
["Type of DNA or RNA used in the experiment",null,"Whether the source material was selected for certain properties (for\nexample methyl filtrated)",null,"the sequencing technique intended for the library."]
["library source","library source explanation","library selection","library selection explanation","Library strategy","Library strategy explanation"]
["GENOMIC"," Genomic DNA (includes PCR products from genomic DNA).","5-methylcytidine antibody"," Selection of methylated DNA fragments using an antibody raised against 5-methylcytosine or 5-methylcytidine (m5C).","AMPLICON"," Sequencing of overlapping or distinct PCR or RT-PCR products. For example, metagenomic community profiling using SSU rRNA."]
["GENOMIC SINGLE CELL","No description","CAGE"," Cap-analysis gene expression.","ATAC-seq"," Assay for Transposase-Accessible Chromatin (ATAC) strategy is used to study genome-wide chromatin accessibility. alternative method to DNase-seq that uses an engineered Tn5 transposase to cleave DNA and to integrate primer DNA sequences into the cleaved genomic DNA."]
["METAGENOMIC"," Mixed material from metagenome.","cDNA"," PolyA selection or enrichment for messenger RNA (mRNA); synonymize with PolyA","Bisulfite-Seq"," MethylC-seq. Sequencing following treatment of DNA with bisulfite to convert cytosine residues to uracil depending on methylation status."]
["METATRANSCRIPTOMIC"," Transcription products from community targets","cDNA_oligo_dT","No description","ChIA-PET"," Direct sequencing of proximity-ligated chromatin immunoprecipitates."]
["SYNTHETIC"," Synthetic DNA.","cDNA_randomPriming","No description","ChIP-Seq"," ChIP-seq, Chromatin ImmunoPrecipitation, reveals binding sites of specific proteins, typically transcription factors (TFs) using antibodies to extract DNA fragments bound to the target protein."]
["TRANSCRIPTOMIC"," Transcription products or non genomic DNA (EST, cDNA, RT-PCR, screened libraries).","ChIP"," Chromatin immunoprecipitation","CLONE"," Genomic clone based (hierarchical) sequencing."]
["TRANSCRIPTOMIC SINGLE CELL","No description","ChIP-Seq"," Chromatin immunoPrecipitation, reveals binding sites of specific proteins, typically transcription factors (TFs) using antibodies to extract DNA fragments bound to the target protein.","CLONEEND"," Clone end (5’, 3’, or both) sequencing."]
["VIRAL RNA"," Viral RNA.","DNase"," DNase I endonuclease digestion and size selection reveals regions of chromatin where the DNA is highly sensitive to DNase I.","CTS"," Concatenated Tag Sequencing"]
["OTHER"," Other, unspecified, or unknown library source material.","HMPR"," Hypo-methylated partial restriction digest","DNase-Hypersensitivity"," Sequencing of hypersensitive sites, or segments of open chromatin that are more readily cleaved by DNaseI."]
[null,null,"Hybrid Selection"," Selection by hybridization in array or solution.","EST"," Single pass sequencing of cDNA templates"]
[null,null,"Inverse rRNA"," depletion of ribosomal RNA by oligo hybridization.","FAIRE-seq"," Formaldehyde Assisted Isolation of Regulatory Elements. Reveals regions of open chromatin."]
[null,null,"Inverse rRNA selection"," depletion of ribosomal RNA by inverse oligo hybridization.","FINISHING"," Sequencing intended to finish (close) gaps in existing coverage."]
[null,null,"MBD2 protein methyl-CpG binding domain"," Enrichment by methyl-CpG binding domain.","FL-cDNA"," Full-length sequencing of cDNA templates"]
[null,null,"MDA"," Multiple Displacement Amplification, a non-PCR based DNA amplification technique that amplifies a minute quantifies of DNA to levels suitable for genomic analysis.","Hi-C"," Chromosome Conformation Capture technique where a biotin-labeled nucleotide is incorporated at the ligation junction, enabling selective purification of chimeric DNA ligation junctions followed by deep sequencing."]
[null,null,"MF"," Methyl Filtrated","MBD-Seq"," Methyl CpG Binding Domain Sequencing."]
[null,null,"MNase"," Identifies well-positioned nucleosomes. uses Micrococcal Nuclease (MNase) is an endo-exonuclease that processively digests DNA until an obstruction, such as a nucleosome, is reached.","MeDIP-Seq"," Methylated DNA Immunoprecipitation Sequencing."]
[null,null,"MSLL"," Methylation Spanning Linking Library","miRNA-Seq"," Micro RNA sequencing strategy designed to capture post-transcriptional RNA elements and include non-coding functional elements. (see pubmed 21787409 for details)"]
[null,null,"Oligo-dT"," enrichment of messenger RNA (mRNA) by hybridization to Oligo-dT.","MNase-Seq"," Identifies well-positioned nucleosomes. uses Micrococcal Nuclease (MNase) is an endo-exonuclease that processively digests DNA until an obstruction, such as a nucleosome, is reached."]
[null,null,"padlock probes capture method"," Targeted sequence capture protocol covering an arbitrary set of nonrepetitive genomics targets. An example is capture bisulfite sequencing using padlock probes (BSPP).","MRE-Seq"," Methylation-Sensitive Restriction Enzyme Sequencing."]
[null,null,"PCR"," target enrichment via PCR","ncRNA-Seq"," Capture of other non-coding RNA types, including post-translation modification types such as snRNA (small nuclear RNA) or snoRNA (small nucleolar RNA), or expression regulation types such as siRNA (small interfering RNA) or piRNA/piwi/RNA (piwi-interacting RNA)."]
[null,null,"PolyA"," PolyA selection or enrichment for messenger RNA (mRNA); should replace cDNA enumeration.","POOLCLONE"," Shotgun of pooled clones (usually BACs and Fosmids)."]
[null,null,"RACE"," Rapid Amplification of cDNA Ends.","RAD-Seq","No description"]
[null,null,"RANDOM"," No Selection or Random selection","RIP-Seq"," Direct sequencing of RNA immunoprecipitates (includes CLIP-Seq, HITS-CLIP and PAR-CLIP)."]
[null,null,"RANDOM PCR"," Source material was selected by randomly generated primers.","RNA-Seq"," Random sequencing of whole transcriptome, also known as Whole Transcriptome Shotgun Sequencing, or WTSS). (see pubmed 18611170 for details)"]
[null,null,"Reduced Representation"," Reproducible genomic subsets, often generated by restriction fragment size selection, containing a manageable number of loci to facilitate re-sampling.","SELEX"," Systematic Evolution of Ligands by Exponential enrichment"]
[null,null,"repeat fractionation"," Selection for less repetitive (and more gene rich) sequence through Cot filtration (CF) or other fractionation techniques based on DNA kinetics.","ssRNA-seq"," Strand-specific RNA sequencing."]
[null,null,"Restriction Digest"," DNA fractionation using restriction enzymes.","Synthetic-Long-Read"," binning and barcoding of large DNA fragments to facilitate assembly of the fragment"]
[null,null,"RT-PCR"," target enrichment via","Targeted-Capture"," Enrichment of a targeted subset of loci."]
[null,null,"size fractionation"," Physical selection of size appropriate targets.","Tethered Chromatin Conformation Capture"]
[null,null,"other"," Other library enrichment, screening, or selection process.","Tn-Seq"," Quantitatively determine fitness of bacterial genes based on how many times a purposely seeded transposon gets inserted into each gene of a colony after some time."]
[null,null,"unspecified"," Library enrichment, screening, or selection is not specified.","VALIDATION","  CGHub special request:  Independent experiment to re-evaluate putative variants."]
[null,null,null,null,"WCS"," Random sequencing of a whole chromosome or other replicon isolated from a genome."]
[null,null,null,null,"WGA"," Whole Genome Amplification followed by random sequencing. (see pubmed 1631067,8962113 for details)"]
[null,null,null,null,"WGS"," Whole Genome Sequencing - random sequencing of the whole genome (see pubmed 10731132 for details)"]
[null,null,null,null,"WXS"," Random sequencing of exonic regions selected from the genome. (see pubmed 20111037 for details)"]
[null,null,null,null,"OTHER"," Library strategy not listed."]

{"sheet":"Version","index":2,"n_rows":7,"n_cols":3,"rows_kept":7,"cut":false,"hidden":false,"merged":[]}
["Date","Quoi","Qui"]
["2023-04-12T22:34:00"]
["2023-08-07T18:03:00","ajout infos pour metadata ENA (lib, espèce)","Gérald"]
["2023-08-24T19:12:00","Uniformisation cellules genome et transcriptome","Gérald"]
["2023-09-25T11:15:00","pré-remplissage position","Gérald + Camille M."]
["2023-09-29T15:37:00","précision remplir les infos de lib si RTL","Gérald"]
["2025-02-28T08:08:00","passage à 2784 echantillons\nAjout info pour conserver les échantillons ou non","Gérald"]

{"sheet":"Feuil1","index":3,"n_rows":0,"n_cols":0,"rows_kept":0,"cut":false,"hidden":true,"merged":[]}